본사 판매 정책에 따라 서울/경기 일부 지역에서만 구매가 가능합니다. |
SYBR Green이나 hydrolysis probe를 이용한 miRCURY LNA miRNA PCR System에 최적화된 cDNA 합성 kit입니다.
| 제품번호 | 제품명 | 용량 |
| 339340 | miRCURY LNA RT Kit | 8-64 rxn |
*실시간 판매로 인하여 재고가 소진될 수 있습니다.
*재고 보유시 1-2일, 수입 발주시 5-6주 정도 소요됩니다.
| * 구매 관련 문의 T.031-728-3256 * 모든 제품은 부가세가 포함된 금액입니다. |
|
■ Components
| 제품명 | miRCURY LNA RT Kit | Description |
| 제품번호 | 339340 (8-64 rxn)* | |
| 5x miRCURY RT SYBR® Green Reaction Buffer,† including Mg2+, SYBR® Green RT primer, and dNTPs | 128 µl | Buffer optimized for Poly(A) polymerization and reverse transcription; contains a universal reverse transcription primer for the SYBR® Green-based workflow, Mg2+, and dNTPs. |
| 5x miRCURY RT Probe Reaction Buffer, † including Mg2+, Probe RT primer and dNTPs | 128 µl | Buffer optimized for Poly(A) polymerization and reverse transcription; contains a universal reverse transcription primer for the Probe-based workflow, Mg2+, and dNTPs |
| 10x miRCURY RT Enzyme Mix | 64 µl | An optimized blend of Reverse Transcription Enzyme and Poly(A) polymerase developed for use in real-time, two-step PCR: ▷ HotStarRT-Script Reverse Transcriptase (a modified form of a recombinant 77 kDa reverse transcriptase) ▷ E. coli Poly(A) Polymerase |
| UniSp6 RNA Spike-in Template, dried down | 12 fmol | Synthetic transcript for monitoring successful reverse transcription |
| Nuclease-free water | 1.5 ml | Ultrapure quality, PCR-grade water |
* Depending on the reaction volumes used (10-80 µl), there are sufficient reagents in the kit for 8-64 reactions.
† The number of standard reactions is based on a reaction volume of 20 µl containing 10 pg – 5 µg total RNA.
■ Equipment and Reagents to Be Supplied by User
▷ Nuclease-free plastic tubes (for 20 µl reactions)
▷ Nuclease-free PCR tubes or plates for use with individual assays
▷ Nuclease-free, aerosol-barrier pipette tips
▷ Ice
▷ PCR cycler, heating block or water bath (capable of reaching 95°C)
▷ Vortexer
▷ Microcentrifuge and plate centrifuge
▷ Sealing foils for PCR plates
▷ Real-time PCR instrument
■ Storage: –30 to –15°C in a constant-temperature freezer (자세한 내용은 핸드북 참조)
■ 제품 주의사항
▷ miRCURY LNA miRNA (probe) PCR Assay 및 Panel, miRCURY LNA SYBR Green (probe) PCR Kit와 함께 사용하는 제품입니다.
▷ miRCURY LNA miRNA PCR System 제품을 모두 사용해야 결과에 대한 개런티가 가능합니다.
제품 자세히 보기(QIAGEN 홈페이지) Kit hand book 다운로드
| * 구매 관련 문의 T.031-728-3256 * 모든 제품은 부가세가 포함된 금액입니다. |
|
■ Components
| 제품명 | miRCURY LNA RT Kit | Description |
| 제품번호 | 339340 (8-64 rxn)* | |
| 5x miRCURY RT SYBR® Green Reaction Buffer,† including Mg2+, SYBR® Green RT primer, and dNTPs | 128 µl | Buffer optimized for Poly(A) polymerization and reverse transcription; contains a universal reverse transcription primer for the SYBR® Green-based workflow, Mg2+, and dNTPs. |
| 5x miRCURY RT Probe Reaction Buffer, † including Mg2+, Probe RT primer and dNTPs | 128 µl | Buffer optimized for Poly(A) polymerization and reverse transcription; contains a universal reverse transcription primer for the Probe-based workflow, Mg2+, and dNTPs |
| 10x miRCURY RT Enzyme Mix | 64 µl | An optimized blend of Reverse Transcription Enzyme and Poly(A) polymerase developed for use in real-time, two-step PCR: ▷ HotStarRT-Script Reverse Transcriptase (a modified form of a recombinant 77 kDa reverse transcriptase) ▷ E. coli Poly(A) Polymerase |
| UniSp6 RNA Spike-in Template, dried down | 12 fmol | Synthetic transcript for monitoring successful reverse transcription |
| Nuclease-free water | 1.5 ml | Ultrapure quality, PCR-grade water |
* Depending on the reaction volumes used (10-80 µl), there are sufficient reagents in the kit for 8-64 reactions.
† The number of standard reactions is based on a reaction volume of 20 µl containing 10 pg – 5 µg total RNA.
■ Equipment and Reagents to Be Supplied by User
▷ Nuclease-free plastic tubes (for 20 µl reactions)
▷ Nuclease-free PCR tubes or plates for use with individual assays
▷ Nuclease-free, aerosol-barrier pipette tips
▷ Ice
▷ PCR cycler, heating block or water bath (capable of reaching 95°C)
▷ Vortexer
▷ Microcentrifuge and plate centrifuge
▷ Sealing foils for PCR plates
▷ Real-time PCR instrument
■ Storage: –30 to –15°C in a constant-temperature freezer (자세한 내용은 핸드북 참조)
■ 제품 주의사항
▷ miRCURY LNA miRNA (probe) PCR Assay 및 Panel, miRCURY LNA SYBR Green (probe) PCR Kit와 함께 사용하는 제품입니다.
▷ miRCURY LNA miRNA PCR System 제품을 모두 사용해야 결과에 대한 개런티가 가능합니다.
