본사 판매 정책에 따라 서울/경기 일부 지역에서만 구매가 가능합니다. |
세포, 조직, 효모 샘플에서 total RNA 분리/ 정제가 가능한 QIAGEN 대표적인 RNA 추출 키트입니다. RNeasy Micro kit는 적은 양의 tissue나 1 cell부터 RNA를 추출할 수 있는 제품입니다.
| 제품번호 | 제품명 | 용량 |
| 74004 | RNeasy Micro Kit | 50 rxn |
*실시간 판매로 인하여 재고가 소진될 수 있습니다.
*재고 보유시 1-2일, 수입 발주시 5-6주 정도 소요됩니다.
| * 구매 관련 문의 T.031-728-3256 * 모든 제품은 부가세가 포함된 금액입니다. |
|
■ Specification
| Applications | RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis |
| Elution volume | 10-14 µl |
| Format | Spin column |
| Main sample type | Tissue, cells, |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA,poly A+ mRNA, DNA or protein | Total RNA |
| Sample amount | < 5 mg tissue, < 5 x 10^5 cells |
| Technology | Silica technology |
| Time per run or per prep | 30-40 minutes |
| Yield | Varies |
■ Components
| 제품명 | RNeasy Micro Kit |
| 제품번호 | 74004 (50rxn) |
| RNeasy MinElute Spin Columns | 50 |
| Collection Tubes (1.5 ml) | 50 |
| Collection Tubes (2 ml)* | 100 |
| Buffer RLT† | 45 ml |
| Buffer RW1† | 45 ml |
| Buffer RPE‡ (concentrate) | 11 ml |
| RNAse-Free Water | 3 x 10 ml |
| Carrier RNA, poly-A | 310 ug |
| RNase-Free Set | RNase-Free DNase I (lyophilized) 1500 units Buffer RDD 2 x 2 ml RNase-Free Water 1.5 ml |
† Contains a guanidine salt. Not compatible with disinfectants containing bleach.
‡ Before using for the first time, add 4 volumes of ethanol (96–100%) as indicated on the bottle to obtain a working solution.
■ Equipment and Reagents to Be Supplied by User
- For all protocols
▷ 14.3 M β-mercaptoethanol (β-ME) (commercially available solutions are usually 14.3 M)
▷ Ethanol (70% and 96-100%)
▷ Sterile, RNase-free pipet tips
▷Microcentrifuge (with rotor for 2 ml tubes)
▷Vortexer
▷Disposable gloves
▷Reagent for RNA stabilization
-> For cell samples: RNAprotect Cell Reagent or liquid nitrogen
-> For tissue samples: RNAprotect Tissue Reagent, Allprotect Tissue Reagent, or liquid nitrogen
▷ Equipment for sample disruption and homogenization: Trypsin and PBS, QIAshredder homogenizer, TissueRuptor, TissueLyser
-For RNA purification from heart, muscle, and skin tissue
▷ QIAGEN Proteinase K (>600 mAU/ml, solution)
▷ Heating block or water bath capable of reaching 55°C
* 샘플 및 실험 방법에 따라 자세한 내용은 hand book을 확인 해주세요.
제품 자세히 보기(QIAGEN 홈페이지) Kit hand book(74004) 다운로드
| * 구매 관련 문의 T.031-728-3256 * 모든 제품은 부가세가 포함된 금액입니다. |
|
■ Specification
| Applications | RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis |
| Elution volume | 10-14 µl |
| Format | Spin column |
| Main sample type | Tissue, cells, |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA,poly A+ mRNA, DNA or protein | Total RNA |
| Sample amount | < 5 mg tissue, < 5 x 10^5 cells |
| Technology | Silica technology |
| Time per run or per prep | 30-40 minutes |
| Yield | Varies |
■ Components
| 제품명 | RNeasy Micro Kit |
| 제품번호 | 74004 (50rxn) |
| RNeasy MinElute Spin Columns | 50 |
| Collection Tubes (1.5 ml) | 50 |
| Collection Tubes (2 ml)* | 100 |
| Buffer RLT† | 45 ml |
| Buffer RW1† | 45 ml |
| Buffer RPE‡ (concentrate) | 11 ml |
| RNAse-Free Water | 3 x 10 ml |
| Carrier RNA, poly-A | 310 ug |
| RNase-Free Set | RNase-Free DNase I (lyophilized) 1500 units Buffer RDD 2 x 2 ml RNase-Free Water 1.5 ml |
† Contains a guanidine salt. Not compatible with disinfectants containing bleach.
‡ Before using for the first time, add 4 volumes of ethanol (96–100%) as indicated on the bottle to obtain a working solution.
■ Equipment and Reagents to Be Supplied by User
- For all protocols
▷ 14.3 M β-mercaptoethanol (β-ME) (commercially available solutions are usually 14.3 M)
▷ Ethanol (70% and 96-100%)
▷ Sterile, RNase-free pipet tips
▷Microcentrifuge (with rotor for 2 ml tubes)
▷Vortexer
▷Disposable gloves
▷Reagent for RNA stabilization
-> For cell samples: RNAprotect Cell Reagent or liquid nitrogen
-> For tissue samples: RNAprotect Tissue Reagent, Allprotect Tissue Reagent, or liquid nitrogen
▷ Equipment for sample disruption and homogenization: Trypsin and PBS, QIAshredder homogenizer, TissueRuptor, TissueLyser
-For RNA purification from heart, muscle, and skin tissue
▷ QIAGEN Proteinase K (>600 mAU/ml, solution)
▷ Heating block or water bath capable of reaching 55°C
* 샘플 및 실험 방법에 따라 자세한 내용은 hand book을 확인 해주세요.
