한 번에 많은 양의 cell, tissue에서 RNA를 추출하실 때 샘플을 나눠 실험하셔 불편하셨나요?
대용량 Kit가 없어 QIAzol 등 매뉴얼 방식으로 실험하시느라 불편하셨나요?
간편한 spin column 방식을 이용해 RNA를 추출할 수 있는
QIAGEN의 RNeasy Maxi kit를 소개합니다!
샘플 종류에 따른 평균 RNA 추출 농도
| Animal cells | Mouse tissue | Yeast cells |
| LMH | HeLa | COS-7 | Lymphocytes
(unstimulated) | Liver | Lung | Spleen | S. cerevisiae |
| Amount of sample | 5 x 108 | 4 x 108 | 1.8 x 108 | 5 x 108 | 1 g | 0.5 g | 1 g | 1 x 109 |
Average
yield* | 5.7 mg | 6.0 mg | 5.8 mg | 0.3 mg | 3.6 mg | 0.6 mg | 3.2 mg | 2.4 mg |
* Elution volume: 800-2400 ul
* 소요시간: 약 1시간
RNeasy Maxi Kit의 spin column capacity
| Sample capacity(amount) | Binding capacity |
| RNeay Maxi Kit | 5x107 - 5x108 cells; 150 mg-1 g tissue | 6 mg RNA |
믿을 수 있는 결과!
| 
|
High-quality RNA from a variety of samples. Tissue (mouse), cell, yeast 샘플을 RNeasy Maxi Kit로 RNA 추출한 결과.
| RNA 추출 과정에서 효과적인 genomic DNA 제거 Total RNA was isolated from the indicated number of HeLa cells using the RNeasy Micro Kit. Quantitative, real-time PCR was carried out on an ABI Sequence Detection System using the QuantiTect Probe PCR Kit, with primers and probes specific for c-jun. In order to evaluate the efficiency of the on-column DNase digestion, each sample was amplified after reverse transcription (+RT) and directly, without a reverse transcription step (-RT). The lack of signal in the "-RT" samples indicates that no DNA was detectable. Of the "-RT" results, only the 50,000 cell data points are visible.
|
Ordering information
| Product | Cat no. | Reaction | Description |
| RNeasy Maxi Kit | 75162 | 12 | 대량 샘플에서 total RNA를 spin column 형식으로 추출 가능!
- Cell: 5x107 - 5x108 cells
- Tissue: 150 mg-1 g
|
*제품문의 T. 031-728-3239, E. technical@dawinbiotech.com
#QIAGEN #다윈바이오 #RNA #Cell #Tissue #Yeast #Purification
한 번에 많은 양의 cell, tissue에서 RNA를 추출하실 때 샘플을 나눠 실험하셔 불편하셨나요?
대용량 Kit가 없어 QIAzol 등 매뉴얼 방식으로 실험하시느라 불편하셨나요?
간편한 spin column 방식을 이용해 RNA를 추출할 수 있는
QIAGEN의 RNeasy Maxi kit를 소개합니다!
샘플 종류에 따른 평균 RNA 추출 농도
(unstimulated)
yield*
* Elution volume: 800-2400 ul
* 소요시간: 약 1시간
RNeasy Maxi Kit의 spin column capacity
믿을 수 있는 결과!
High-quality RNA from a variety of samples.
Tissue (mouse), cell, yeast 샘플을 RNeasy Maxi Kit로 RNA 추출한 결과.
RNA 추출 과정에서 효과적인 genomic DNA 제거
Total RNA was isolated from the indicated number of HeLa cells using the RNeasy Micro Kit. Quantitative, real-time PCR was carried out on an ABI Sequence Detection System using the QuantiTect Probe PCR Kit, with primers and probes specific for c-jun. In order to evaluate the efficiency of the on-column DNase digestion, each sample was amplified after reverse transcription (+RT) and directly, without a reverse transcription step (-RT). The lack of signal in the "-RT" samples indicates that no DNA was detectable. Of the "-RT" results, only the 50,000 cell data points are visible.
Ordering information
- Cell: 5x107 - 5x108 cells
- Tissue: 150 mg-1 g
*제품문의 T. 031-728-3239, E. technical@dawinbiotech.com
#QIAGEN #다윈바이오 #RNA #Cell #Tissue #Yeast #Purification