Counting cells using a hemocytometer
Protocol to obtain a viable cell count from suspension cells using a hemocytometer.
Preparing cell suspension
To calculate the number of viable cells/mL:
The final value is the number of viable cells/mL in the original cell suspension.
To calculate viability:
If both live and dead cell counts have been recorded for each set of 16 corner squares, an estimate viability can be calculated.
Figure 1. Hemocytometer gridlines.
Hemocytometer diagram indicating one of the sets of 16 squares that should be used for counting.