Ni-NTA Affinity Resin
Ni-NTA Affinity Resin은 native 또는 denaturing 조건의 cell lysate에서 간단하고 신속하게 His tag이 결합되어 있는 재조합 단백질 정제를 위한 제품입니다. Affinity chromatography를 수행하기 위하여 핸드북에 제공된 신속한 정제 프로토콜을 사용하면 재조합 단백질을 몇 분 안에 회수할 수 있습니다.
Key benefits
- One step purification
- High capacity : 50 mg/ml
- Purification under native or denaturing conditions
- Minimum metal leaching
Characteristics of the resin
Supporting matrix : Highly cross-lined 6% agarose
Charged metal ion : Ni2+
Bead size range : 45-165 μm
Recommended working pH : pH 2.0-12.0
Static binding capacity : >40 mg 6x His-tagged
Maximum pressure : 0.3 MPa (3 bar)
Chemical stability : High
Solubility in water : Insoluble
Ordering Information
Cat no | Product Name | Binding Capacity | Medium (ml) | Slurry (ml) |
| Ni-NTA Affinity Resin - Amintra | > 40mg 6x His-tagged | 10 ml | 20 ml |
25 ml | 55 ml |
100 ml | 200 ml |
Related products
Category | Cat no. | Product | Size | Application |
Resin | ab270018 | Cobalt NTA Affinity Resin - Amintra | 10 ml, 25 ml, 100 ml | His-tagged recombinant protein purification |
ab270529 | His-Tag Protein Purification Column | Pre-packed, 5 x 5 ml | His-tagged recombinant protein purification |
ab270528 | His-Tag Protein Purification Column | Pre-packed, 5 x 1 ml | His-tagged recombinant protein purification |
Antibody | ab18184 | Anti-6X His tag® antibody [HIS.H8] | 100 ug | ICC/IF, ELISA, Dot blot, WB, IP, EMSA |
ab1187 | Anti-6X His tag® antibody (HRP) | 100 ug | ELISA, WB |
ab9108 | Anti-6X His tag® antibody | 100 ug | IP, WB, IHC-P, ICC/IF, ELISA, ChIP, Flow Cyt |
ab1206 | Anti-6X His tag® antibody (FITC) | 250 ug | ELISA, WB, Flow Cyt |
ab213204 | Anti-6X His tag® antibody [EPR20547] | 100 ul | WB, ICC/IF, IP, Flow Cyt, ChIP, IHC-P |
ELISA kit | ab128573 | GST 6XHis-tag ELISA Kit | | ELISA |
Protein | ab14943 | 6X His tag® peptide | 100 ug, 500 ug | Blocking |
학술 문의 Tel. 031-728-3233, 3237 E-mail : technical@dawinbio.com 카카오톡 플러스친구 ID: abcam
Ni-NTA Affinity Resin
Ni-NTA Affinity Resin은 native 또는 denaturing 조건의 cell lysate에서 간단하고 신속하게 His tag이 결합되어 있는 재조합 단백질 정제를 위한 제품입니다. Affinity chromatography를 수행하기 위하여 핸드북에 제공된 신속한 정제 프로토콜을 사용하면 재조합 단백질을 몇 분 안에 회수할 수 있습니다.
Key benefits
- One step purification
- High capacity : 50 mg/ml
- Purification under native or denaturing conditions
- Minimum metal leaching
Characteristics of the resin
Supporting matrix : Highly cross-lined 6% agarose
Charged metal ion : Ni2+
Bead size range : 45-165 μm
Recommended working pH : pH 2.0-12.0
Static binding capacity : >40 mg 6x His-tagged
Maximum pressure : 0.3 MPa (3 bar)
Chemical stability : High
Solubility in water : Insoluble
Ordering Information
Related products
학술 문의 Tel. 031-728-3233, 3237 E-mail : technical@dawinbio.com 카카오톡 플러스친구 ID: abcam