[ QIAGEN ]QIAGEN RNA purification kits

2020-06-19
조회수 6557

■ 조직이나 세포에서 total RNA purification을 위한 QIAGEN의 대표적인 제품 

ProductRNeasy Micro KitRNeasy Mini KitRNeasy Midi KitRNeasy Maxi Kit
Cat no.74004(50)74104(50) / 74106(250)75144(50)75162(12)
ApplicationsNorthern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis,
next-generation sequencing
PCR, qPCR, real-time RT-PCR, microarray
Elution volume10–14 µl30–100 µl300–500 µl800–2400 µl
FormatSpin column
Main sample typeTissue, cellsTissue, cellsTissue, cellsTissue, cells
ProcessingManualManual (centrifugation)ManualManual
Sample amount<5 mg0.5–30 mg20–250 mg150 mg – 1 g
TechnologySilica technologySilica technologySilica technologySilica technology
Time per run30–40 minutes20 minutes<1 hour1 hour
YieldVariesVariesVariesVaries


 - kit에 gDNA removal reagent를 포함하고 있는 제품 

ProductRNeasy Plus Micro KitRNeasy Plus Mini KitRNeasy Plus Universal Mini Kits
Cat no.74034(50)74134(50) / 74136(250)73404(50)
ApplicationsNorthern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR,
array analysis, next-generation sequencing
Elution volume14 µl30-50 µl30µl
FormatSpin column
Main sample typeTissue, cellsTissue, cellsTissue samples including difficult-to-lyse and
demanding tissues
ProcessingManual (centrifugation)Manual (centrifugation)Manual (centrifugation), automated (QIAcube)
Sample amount5 x 10e5 cells or
5 mg tissue
30 mg (<700 µl)5-50 mg
TechnologySilica technologySilica technologyQIAzol/Spin column technology
Time per run25 min25 minutes
YieldVariesVariesVaries
Integrated removal of genomic DNAYesYesYes


- Ultra-Clean Production (UCP)을 위한 spin column과 buffer가 포함된 제품

ProductRNeasy UCP Micro Kit
Cat no.73934(50)
ApplicationsNGS, end-point RT-PCR, qPCR, array analysis
Elution volume10-20 µl 
FormatSpin column
Main sample typeTissue, cells, low biomass samples
ProcessingManual (centrifugation)
Sample amount<5 mg tissue or 5 x 10e5 cells
TechnologySilica technology  / Ultra-clean production
Time per run30 - 40 min
YieldVaries


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