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AbcamTissue clearing reagents and kits

2019-07-19

Tissue clearing reagents and kits

Recently developed tissue clearing methods, such as CLARITY, CUBIC, i/3/u/vDISCO, etc, have been used to produce beautiful images that push the boundaries in fields such as neuroscience and developmental biology. To streamline this process, we have developed tissue clearing kits and reagents so that you can quickly clear tissues or 3D cell cultures. The protocols are simple, use standard laboratory equipment, and are compatible with immunostaining, fluorescent proteins, and chemical dyes. Clearing is reversible so you can section your tissue for conventional H&E or IHC staining after 3D imaging.

The tissue clearing reagents developed with Visikol Inc, who are experts in digital pathology, 3D cell culture assays and 3D tissue imaging, and developed the Visikol® HISTO™ tissue clearing technology which is the basis of our reagents.

You can get tissue clearing kits validated with antibodies for essential markers, such as NeuN, GFAP, and Iba1. We test these antibodies on 1 mm mouse brain sections, neuronal spheroids, and hepatic spheroids (depending on the marker).



Tissue Clearing Kit ab243298 enables the easy set-up of rapid tissue clearing for 3D tissue imaging using simple protocols and standard laboratory equipment.

The kit enables you to set up tissue clearing easily and clear tissue quickly. The protocols are simple and use standard laboratory equipment, and can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.


Components30 ml15 ml
Tissue Clearing Antibody Buffer1 x 30ml1 x 15ml
Tissue Clearing Blocking Buffer1 x 30ml1 x 15ml
Tissue Clearing Penetration Buffer1 x 30ml1 x 15ml
Tissue Clearing Permeabilization Buffer1 x 30ml1 x 15ml
Tissue Clearing Reagent 11 x 30ml1 x 15ml
Tissue Clearing Reagent 21 x 30ml1 x 15ml
Tissue Clearing Washing Buffer 10X1 x 70ml1 x 35ml



3D Cell Culture Clearing Kit ab243299 enables the easy set-up of rapid 3D cell culture clearing for 3D imaging using simple protocols and standard laboratory equipment.

This kit can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.


Components30 ml15 ml
3D Cell Culture Clearing Reagent1 x 30ml1 x 15ml
Tissue Clearing Antibody Buffer1 x 30ml1 x 15ml
Tissue Clearing Blocking Buffer1 x 30ml1 x 15ml
Tissue Clearing Penetration Buffer1 x 30ml1 x 15ml
Tissue Clearing Washing Buffer 10X1 x 70ml1 x 35ml
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