[Merck]Genomics Workflow 제품 선택

조회수 137









Molecular Cloning, Expression

Cloning, Expression

Cat.No.DescriptionUnitList price
69864-3CNpET-28a(+) DNA - Novagen10 μg429,500
71146-3pETDuet-1 DNA - Novagen10 μg677,100
TR-1003-GPolybrene Transfection Reagent1 mL85,800
L2542-500MLLB Broth (Miller)500 mL64,900
M6030-1KGM9, Minimal Salts, 5X1 kg491,100
71754-3Terrific Broth - Novagen500 g164,500
S1797-100MLSOC Medium100 mL289,500
Y1625-1KGYeast Extract1 kg547,700
L3022-1KGLB Broth (Lennox)1 kg289,500






Transformation - Competent Cells

Cat.No.DescriptionUnitList price
69450-4CNBL21(DE3) Competent Cells - Novagen1 mL407,700
69451-4CNBL21(DE3)pLysS Competent Cells - Novagen1 mL427,500




Transfection

• Eukaryotic cells, insect cells에 Transfection 최적화
  Ex>Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080,

  K-562, RAW 264.7, Jurkat, PC-12, HCT 116



Cat.No.DescriptionUnitList price
6366546001X-tremeGENE™ HP DNA Transfection Reagent5 x 1 mL3,424,200
6365809001X-tremeGENE™ 9 DNA Transfection Reagent5 x 1 mL3,163,000
70967-3CNGeneJuice® Transfection Reagent1 mL740,200






DNA, RNA Sample Preparation

GenElute™ - E Single Spin Technology

• Negative chromatography 방법을 이용하여 단 한 번의 centrifuge로
  원하는 nucleic acid 추출 가능

• Ethanol이 들어간 washing buffer를 사용하지 않아 PCR contaminant가 포함될 우려가 없음




Cat.No.DescriptionUnitList price
T9424-200MLTRI Reagent®200 mL807,300
R0901-500MLRNAlater®500 mL1,068,800
P2069-400MLPhenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with
10 mM Tris, pH 8.0, 1 mM EDTA
400 mL645,900
EC600-250RXNGenElute™-E DNA Cleanup Kit250 reactions1,397,200
EC300-250RXNGenElute™-E Single Spin Tissue DNA Kit250 reactions1,824,000
P2308-100MGProteinase K from Tritirachium album100 mg338,800
EC100-250RXNGenElute™-E Single Spin Blood DNA Kit250 reactions1,522,400
11667327001DNA Isolation Kit for Mammalian Blood25 reactions345,600
11814770001DNA Isolation Kit for Cells and Tissues10 isolations362,300
NA0300-1KTGenElute™ HP Plasmid Maxiprep Kit10 purifications405,400
NA9604-1KTGenElute™ HP 96-Well Plasmid Miniprep Kit4 x 96 well plates1,902,900
EC800-250RXNGenElute™-E RNA Cleanup Kit250 reactions1,539,600
EC111-100MLGenElute™-E Tissue Stabilizer100 mL258,700









Gene Amplification

Cat.No.DescriptionUnitList price
71975-3KOD Xtreme Hot Start DNA Polymerase200 units792,200
71087-4CNKOD XL DNA Polymerase1250 units2,570,100
D8187-2.5KUJumpStart™ REDTaq® DNA Polymerase2500 units3,840,800
R2523-100RXNREDTaq® ReadyMix™ PCR Reaction Mix100 reactions371,600
P2893-400RXNJumpStart™ Taq ReadyMix™400 reactions1,233,200
KK4602KAPA SYBR® FAST, Universial10 mL569,100
KK4605KAPA SYBR® FAST, ABI PRISM10 mL569,100
KK4611KAPA SYBR® FAST, LightCycler®48010 mL569,100
12140306001GC-RICH PCR System50 reactions462,500
4913949001FastStart Universal Probe Master (Rox)250 x 20 μL190,000
3531287001Transcriptor Reverse Transcriptase200 reactions1,418,900
M1302-40KUM-MLV Reverse Transcriptase4000 units746,700
5893151001Transcriptor Universal cDNA Master100 reactions1,695,500
11483188001First Strand cDNA Synthesis Kit for RT-PCR (AMV)30 reactions758,200
B0300-5VLBetaine solution, 5 M5 vials213,700
AMPD1-1KTDNase I1 kit230,200
M8662-5VLMineral oil5 vials219,300




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