[Merck]Western Blot Workflow

조회수 93



Protein Extraction, Sample Preparation

Lysis, Extraction

  • RIPA : Mammalian cell lysis, protein-protein interaction 연구 목적 사용 가능
  • BugBuster® : Sonication 없이 E. coli의 soluble protein 추출
  • CHAPS : Receptor 또는 membrane protein 추출
  • Lysozyme : Bacteria protein 추출
Cat.No.DescriptionUnitList price (원)
20-188RIPA Lysis Buffer 10 X 100 mL1 L177,900
71456-3BugBuster® Master Mix500 mL400,500
C9426-5GCHAPS hydrate, BioReagent, ≥98% (TLC)5 g568,200
10837059001Lysozyme10 g336,200


Protease Inhibitors

  • Animals, plants, yeast, bacteria, fungi 등 대부분의 조직에서 추출 시 proteolytic activity을 inhibition
  • Serine proteases, cysteine proteases, metalloproteases 등 다양한 protease inhibition
Cat.No.DescriptionUnitList price (원)
4906837001
PhosSTOP™, 20 tablets
20 tablets411,800
11873580001
cOmplete™, EDTA-free Protease Inhibitor Cocktail
20 tablets584,300
11524488001
Pepstatin A, 50 mg
50 mg541,500
P2714-1BTL
Protease Inhibitor Cocktail, for general use, lyophilized powder
1 bottle147,700



Buffer Exchange, Concentration
  • Fast- Single spin으로 빠르게 buffer exchange
  • Gentle- 독특한 구성으로 지속적인 buffer exchange
  • Less buffer- 1.5 mL의 적은 buffer만 필요
Cat.No.DescriptionUnitList price (원)
UFC501024
Amicon® Ultra – 0.5 mL Filters, 24 pack
24 pkg206,200
UFC201024
Amicon® Ultra – 2 mL Filters, 24 pack
24 pkg
261,100
UFC801024
Amicon® Ultra – 4 mL Filters, 24 pack
24 pkg
336,100
UFC901024
Amicon® Ultra – 15 mL Filters, 24 pack
24 pkg
557,200






Electrophoresis


Electrophoresis Reagent, Buffer
Cat.No.DescriptionUnitList price (원)
LUXRGTKIT
mPAGE® Lux Bis-Tris Reagent Kit
1 kt383,000
94964
BLUeye Prestained Protein Ladder
500 μL299,500
MPSTD4
mPAGE® Color Protein Standard
500 μL
243,000
MPSTD3
mPAGE® Unstained Protein Standard
500 μL
176,000
MPSTD2
mPAGE® Western Protein Standard
250 μL
150,500
MPMES
mPAGE® MES SDS Running Buffer Powder
pack of 5 ea
54,400
MPM0PS
mPAGE® MOPS SDS Running Buffer Powder
pack of 5 ea
47,700
MPSB-10ML
mPAGE™ 4X LDS Sample Buffer
10 mL
23,800
A3678-25G
Ammonium persulfate (APS) for electrophoresis, ≥98%
25 g
75,100
T22500-5ML
N,N,N′,N′-Tetramethylethylenediamine (TEMED) ReagentPlus®, 99%
5 mL
52,600
G8898-500G
Glycine, ≥99%
500 g
144,500
T5816-100G
Tricine, cell culture tested, ≥99%
100 g
266,100
RDD008-1KG
Trizma® base, anhydrous, free-flowing, Redi-Dri™, ≥99.9%
1 kg
240,700
L3771-1KG
Sodium dodecyl sulfate ≥98.5%
1 kg
981,400
M3148-25ML
2-Mercaptoethanol suitable for cell culture, 99%
25 mL
48,800
D9163-5G
dl-Dithiothreitol (DTT), ≥99%
5 g
340,300






Precast Protein Gels


mPAGE™ Precast Gel 
  • 기존 electrophoresis tanks에 호환가능
  • 합리적인 가격
  •  중성 pH로 단백질 변형 방지
Cat.No.DescriptionList price (원)
MP41G10
mPAGE™ 4-12% Bis-Tris, 10x8, 10-well
157,500
MP41G12
mPAGE™ 4-12% Bis-Tris, 10x8, 12-well
157,700
MP41G15
mPAGE™ 4-12% Bis-Tris, 10x8, 15-well
157,500
MP42G10
mPAGE™ 4-20% Bis-Tris, 10x8, 10-well157,500
MP42G12
mPAGE™ 4-20% Bis-Tris, 10x8, 12-well
157,700
MP42G15
mPAGE™ 4-20% Bis-Tris, 10x8, 15-well
157,700
MP81G10
mPAGE™ 8-16% Bis-Tris, 10x8, 10-well
157,500
MP81G12
mPAGE™ 8-16% Bis-Tris, 10x8, 12-well
157,500
MP81G15
mPAGE™ 8-16% Bis-Tris, 10x8, 15-well
157,500




Protein Transfer


Transfer Membrane, Blotting Papers 
  • Membrane에 protein의 높은 흡착력으로 강한 단백질 signal
  • 더 긴 유통기한
  • 쉽게 잘라서 사용 가능
Cat.No.DescriptionList price (원)
ISEQ85R
Immobilon®-PSQ PVDF Transfer Membrane, 0.2 μm
607,300
IPVH85R
Immobilon®-P PVDF Transfer Membrane, 0.45 μm
607,300
HATF85R
Immobilon®-NC Transfer Membrane, 0.45 μm
486,800
IMDISP
Immobilon® NOW Dispenser for 8.5 x 10 cm rolls
511,700




Blocking, Antibody Incubation


Loading Control Ab
  • 모든 well에서 로딩된 샘플을 정량화하여 내부 표준으로 활용
  • 각 well마다 단백질 양을 확인하여 통해 관찰 대상의 변화를 설명할 수 있음
Cat.No.DescriptionUnitList price (원)
SAB2108668-100UL
Anti-GAPDH, polyclonal
100 μL
807,500
A2066-100UL
Anti-Actin, polyclonal
100 μL
688,600
SAB4200714-100UL
Anti-HSP70
100 μL
776,000
MAB374
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, Clone 6C5
200 μg
568,800
A5441-.2ML
Anti-β-Actin antibody, Clone AC-15
200 μL
918,300
A2066-100UL
Anti-Actin antibody, Rabbit polyclonal
100 μL
688,600
CB1001-500UG
Anti-GAPDH Mouse mAb (6C5)
500 μg
675,000
G8795-100UL
Anti-GAPDH antibody, Clone GAPDH-71.1
100 μL
868,800
G9545-200UL
Anti-GAPDH antibody, Rabbit polyclonal
200 μL
1,123,700
AB10526
Anti-COX4 (Cytochrome C Oxidase), rabbit polyclonal
100 μg
661,500
07-371
Anti-Histone H2B Antibody
200 μg
741,200
05-1352
Anti-Histone H2B Antibody, Clone 5HH2-2A8
100 μL
786,100
MABE288
Anti-PCNA Antibody, Clone PC10
200 μL
668,200
ABS16
Anti-GAPDH Antibody
100 μg
542,400
AB2302
Anti-GAPDH Antibody
100 μg
661,500



머크의 모든 항체를 한눈에!
머크/씨그마알드리치의 9만종 포트폴리오를 검색하고
실시간 업데이트 되는 할인 및 이벤트 정보를 확인하세요.


Immunodetection Reagent and Consumable
  • 가장 빠른 면역 검출
  • 항체-항원 결합 증가
  • 낮은 Background
Cat.No.DescriptionUnitList price (원)
SNAP2BHMN0100
SNAP i.d.® 2.0 Consumables, Mini Blot Holders

541,900
SNAPABTR
SNAP i.d.® 2.0 Antibody Collection Tray

48,700
SNAP2RL
SNAP i.d.® 2.0 Blot Roller

51,600
SNAP2BHMB050
SNAP i.d.® 2.0 MultiBlot Holders

455,000
SNAP2FRMN01
SNAP i.d.® 2.0 Mini Blot Holding Frame (single pack)

227,900
WBAVDCH01
Immobilon® Block-CH Reagent, Chemiluminescence Detection
500 mL
224,800
WBAVDP001
Immobilon® Block-PO Reagent, Phosphoprotein Detection
500 mL
269,500
T6789-10PAK
Tris-Buffered Saline, with BSA, pH 8.0, powder
10 pack
527,100
91414-10TAB
Tris-Buffered Saline with Tween® 20 detergent, tablets pH 7.6
10 tablet
281,000
T6664-10PAK
Tris-Buffered Saline, pH 8.0, powder
10 pack
134,200
P9416-100ML
Tween® 20 detergent
100 mL
91,600
WBLUF0100
Immobilon® Forte Western HRP Substrate
100 mL
241,400
WBLUR0100
Immobilon® Crescendo Western HRP Substrate
100 mL
192,600
WBKLS0100
Immobilon® Western Chemiluminescent HRP Substrate
2 x 50 mL
205,000
WBULS0100
Immobilon® ECL Ultra Western HRP Substrate
2 x 50 mL
321,800
407207-1KITCN
SignalBoost™ Immunoreaction Enhancer Kit

453,200
T9455-100ML
3,3′,5,5′-Tetramethylbenzidine (TMB), Enhanced HRP Membrane Substrate
100 mL
206,200
2504
ReBlot™ Plus Strong Antibody Stripping Solution
10 x 50 mL
211,600




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