TECHNICAL RESOURCES

AbcamCell viability assay kit

2019-03-08 01:23


Abcam 의 다양한 Cell viability assay kit


Drug 또는 chemical 을 처리한 후 cell viability 확인이 필요하시죠?


Cell viability assay kit 는 cellular metabolism 또는 enzyme activity 와 관련된 drug 를 세포에 처리한 후 cytotoxicity 정도 또는 세포 수의 변화를 확인하고자 할 때 사용하는 기본적인 tool 입니다.


Abcam 에서는 가장 흔하게 사용되는 전통적인 방식의 MTT assay 부터 sensitivity 가 높은 WST-1 assay, 그리고 sensitivity 가 높을 뿐 아니라 세포 독성도 가장 적은 WST-8 assay 까지 다양한 종류의 cell viability assay kit 를 제공하고 있습니다. 이 제품들은 모두 Tetrazolium family 의 chemical 을 기반으로 하고 있습니다. 이 Tetrazolium family 의 checmial 은 살아있는 세포의 mitochondria 에서 생성되는 cellular dehydrogenase 로 인해 색을 지닌 formazan product 를 형성하게 되는데요, 이러한 원리를 이용하여 cell viability 를 OD 값으로 수치화하여 측정 가능합니다.


다양한 종류의 Tetrazolium family



Cytotoxicity test & Cell counting assay 





Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with The Cell Counting Kit 8 (WST-8) (ab228554). HeLa cells at 10,000 cells/well/100 μL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a plate reader.




Cell number was determined with the Cell Counting Kit 8 (WST-8) (ab228554). (A) HeLa cells at 0 to 10,000 cells/well/100 μL, and (B) Jurkat cells at 0 to 100,000 cells/well/100 μL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a plate reader.


Ordering information

Cat no.제품명측정 파장사이즈
ab211091MTT Assay Kit (Cell Proliferation) (ab211091)570 nm1000 tests
ab232856XTT Assay Kit (ab232856)450 nm1 x 96 tests
ab197010MTS Assay Kit (Cell Proliferation) (Colorimetric) (ab197010)490 nm250 tests
500 tests
2500 tests
ab65473WST-1 Assay Kit (Cell Proliferation) (ab65473)440 nm500 tests
2500 tests
ab155902WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902)2500 tests
ab65475WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902)500 tests
2500 tests
ab228554Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)460 nm1000 tests


Cell health assay guide 자세히 보기! click!

* 학술 문의 T. 031-728-3233~4 E. technical@dawinbio.com

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