Reporter Cell LinesPRR이 over expression 혹은 KO 된 reporter cell line

2019-11-14
조회수 4257

 Innate immunity-specific signaling pathway 연구를 위한 Reporter Cell Line


 InvivoGen 세포 활용 연구

     -  특정 유전자의 기능 연구                                          

     -  Cell signaling pathway/signal transduction

     -  Cytokine 생산 확인 

     -  Antibody 기능 검증

     -  PRR(pattern recognition receptor)의 agonist/antagonist 스크리닝 : Toll-like receptors (TLRs), C-type lectin receptors (CLRs), cytosolic 

        DNA sensors (CDSs), aryl hydrocarbon receptor (AhR)

     -  Human(A549, HCT116, HEK293, HeLa, HepG2, HT29, Jurkat, Ramos, THP-1)과 Murine(B16, J774, RAW264.7) 세포를 공급


  두 가지 reporter system 제공

     -   SEAP(secreted embryonic alkaline phosphatase)

     -   Lucia luciferase

     -   InvivoGen의 detection reagent를 이용하여 cell culture supernatant 에서 reporter protein 을 측정합니다.


[ Reporter Cell Lines ]

A549 lung carcinoma : Human A549 lung carcinoma - NF-κB & IRF reporter cells

Cell lineDescriptionCat no.
A549-Dual™ Cells Human NF-κB-SEAP & IRF-Luc Reporter lung carcinomaa549d-nfis
A549-Dual™ KO-MAVS Cells MAVS knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Linea549d-komavs
A549-Dual™ KO-MDA5 Cells MDA-5 knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Linea549d-komda5
A549-Dual™ KO-RIG-I Cells RIG-I knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Linea549d-korigi


B16 melanoma : Murine B16 melanoma - PRR and cytokine reporter cells

Cell lineDescriptionCat no.
B16-Blue™ IFN-α/β Cells Murine B16 melanoma - Type I IFNs reporter cellsbb-ifnt1
B16-Blue™ IFN-γ Cells Murine B16 melanoma - IFNγ reporter cellsbb-ifng
B16-Blue™ ISG Cells Murine B16 melanoma - IFNs reporter cellsbb-ifnabg
B16-Blue™ ISG-KO-STING Cells Murine B16 melanoma - STING Knockout IRF-reporter cellsbb-kostg


HCT116 colon carcinoma : Human HCT116 colorectal carcinoma - NF-κB & IRF reporter cells

Cell lineDescriptionCat no.
HCT116-Dual™ CellsHuman NF-κB-SEAP & IRF-Luc Reporter colorectal carcinomahctd-nfis


HEK293 Reporter Cells : Human embryonic kidney (HEK) 293 cells - PRR or cytokine reporter cells

HEK CLR Reporter Cells CLR SEAP Reporter HEK 293
Cell lineDescriptionCat no.
HEK-Blue™ hDectin-1a Cells Human Dectin-1a / NF-κB / SEAP reporter HEK293 cellshkb-hdect1a
HEK-Blue™ hDectin-1b Cells Human Dectin-1b / NF-κB / SEAP reporter HEK293 cellshkb-hdect1b
HEK-Blue™ mDectin-1b Cells Murine Dectin-1b / NF-κB / SEAP reporter HEK293 cellshkb-mdect1b
HEK-Blue™ mDectin-2 Cells Murine Dectin-2 / NF-κB / SEAP reporter HEK293 cellshkb-mdect2
HEK-Blue™ mMincle Cells Murine Mincle / NF-κB / SEAP reporter HEK293 cellshkb-mmcl
HEK NOD Reporter Cells NOD SEAP Reporter HEK 293
Cell lineDescriptionCat no.
HEK-Blue™ hNOD1 Human NOD1 / NF-kB / SEAP reporter HEK293 cellshkb-hnod1
HEK-Blue™ mNOD1 Murine NOD1 / NF-kB / SEAP reporter HEK293 cellshkb-mnod1
HEK-Blue™ hNOD2 Human NOD2 / NF-kB / SEAP reporter HEK293 cellshkb-hnod2
HEK-Blue™ mNOD2 Murine NOD2 / NF-kB / SEAP reporter HEK293 cellshkb-mnod2
HEK RLR Reporter Cells RLR Lucia Reporter HEK 293
Cell lineDescriptionCat no.
HEK-Lucia™ RIG-I Cells Lucia luciferase reporter HEK293 cells expressing human RIG-Ihkl-hrigi
HEK-Lucia™ Null Cells Lucia luciferase reporter HEK293 cellshkl-null
HEK STING Reporter Cells STING SEAP Reporter 293T
Cell lineDescriptionCat no.
293T-Dual™ hSTING-A162 Cells Dual IRF and IFN-β reporter 293T cells expressing A162 isoform of human STING (S162A)293d-a162
293T-Dual™ hSTING-H232 Cells Dual IRF and IFN-β reporter 293T cells expressing H232 isoform of human STING (R232H)293d-h232
293T-Dual™ hSTING-R232 Cells Dual IRF and IFN-β reporter 293T cells expressing R232 isoform of human STING293d-r232
293T-Dual™ mSTING Cells Dual IRF and IFN-β reporter 293T cells expressing murine STING293d-mstg
293T-Dual™ Null Cells Dual IRF and IFN-β reporter 293T cells293d-null
HEK TLR Reporter Cells TLR SEAP Reporter HEK 293
Cell lineDescriptionCat no.
HEK-Blue™ (NFkB-SEAP) TLR cells

HEK-Blue™ hMD2-CD14 Human MD2-CD14 / NF-κB / SEAP reporter HEK293 cellshkb-hmdcd
HEK-Blue™ hTLR2Human TLR2 / NF-κB / SEAP reporter HEK293 cellshkb-htlr2
HEK-Blue™ hTLR2-TLR1 Cells Human TLR2+TLR1 / NF-κB / SEAP reporter HEK293 cellshkb-htlr21
HEK-Blue™ hTLR2-TLR6 Cells Human TLR2+TLR6 / NF-κB / SEAP reporter HEK293 cellshkb-htlr26
HEK-Blue™ hTLR2 KO-TLR1/6 Cells Human TLR2 (KO-TLR1/6) / NF-κB / SEAP reporter HEK293 cellshkb-htlr2k16
HEK-Blue™ mTLR2 Murine TLR2 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr2
HEK-Blue™ hTLR3 Human TLR3 / NF-κB / SEAP reporter HEK293 cellshkb-htlr3
HEK-Blue™ mTLR3 Murine TLR3 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr3
HEK-Blue™ hTLR4 Human TLR4 / NF-κB / SEAP reporter HEK293 cellshkb-htlr4
HEK-Blue™ mTLR4 Murine TLR4 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr4
HEK-Blue™ hTLR5 Human TLR5 / NF-κB / SEAP reporter HEK293 cellshkb-htlr5
HEK-Blue™ mTLR5 Murine TLR5 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr5
HEK-Blue™ hTLR7 Human TLR7 / NF-κB / SEAP reporter HEK293 cellshkb-htlr7
HEK-Blue™ mTLR7 Murine TLR7 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr7
HEK-Blue™ hTLR8 Human TLR8 / NF-κB / SEAP reporter HEK293 cellshkb-htlr8
HEK-Blue™ mTLR8 Murine TLR8 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr8
HEK-Blue™ hTLR9 Human TLR9 / NF-κB / SEAP reporter HEK293 cellshkb-htlr9
HEK-Blue™ mTLR9 Murine TLR9 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr9
HEK-Blue™ mTLR13 Murine TLR13 / NF-κB / SEAP reporter HEK293 cellshkb-mtlr13
HEK-DUAL™ (NFKB-SEAP / KI-[IL-8]Lucia) TLR CELLS
Cell lineDescriptionCat no.
HEK-Dual™ hTLR2 Human TLR2 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cellshkd-htlr2ni
HEK-Dual™ hTLR3 Human TLR3 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cellshkd-htlr3ni
HEK-Dual™ mTLR4 Murine TLR4 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cellshkd-mtlr4ni
HEK-Dual™ hTLR5 Human TLR5 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cellshkd-htlr5ni
HEK-Dual™ mTLR7 Murine TLR7 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cellshkd-mtlr7ni
HEK-Dual™ hTLR9 Human TLR9 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cellshkd-htlr9ni
HEK-Dual™ Null NF-κB-SEAP / KI-[IL-8]Lucia dual-reporter HEK293 cellshkd-nullni
HEK Cytokine Reporter Cells Cytokines Reporter HEK 293
Interferon Reporter Cells

Cell lineDescriptionCat no.
HEK-Blue™ IFN-α/β Cells Human HEK293 cells - Type I IFNs reporter cellshkb-ifnab
HEK-Blue™ IFN-γ Cells Human HEK293 cells - Type II IFNs reporter cellshkb-ifng
HEK-Dual™ IFN-γ Cells Human IFN-γ SEAP/Luciferase reporter HEK293 cellshkd-ifng
HEK-Blue™ IFN-λ Cells Human HEK293 cells - Type III IFNs reporter cellshkb-ifnl
HEK-Blue™ ISG Cells Interferon Regulatory Factor (IRF)-Inducible SEAP Reporter HEK293 Cellshkb-isg-1
Interleukin Reporter Cells

Cell lineDescriptionCat no.
HEK-Blue™ IL-1β Cells Human & Mouse IL-1β Sensor Cellshkb-il1b
HEK-Blue™ IL-1R Cells Human & Mouse IL-1 Sensor Cellshkb-il1r
HEK-Blue™ IL-2 Cells Human & Mouse IL-2 Reporter Cellshkb-il2
HEK-Blue™ IL-4/IL-13 Cells Human IL-4 / IL-13 SEAP reporter HEK293 cells | JAK/STAT6 pathwayhkb-il413
HEK-Blue™ IL-6 Cells Human IL-6 Sensor Cellshkb-hil6
HEK-Blue™ IL-12 Cells Human & Mouse IL-12 Sensor Cellshkb-il12
HEK-Blue™ IL-17 Cells Human & Mouse IL-17 Reporter Cells, HEK-Blue™ IL-17 Cellshkb-il17
HEK-Blue™ IL-17C Cells Human & Mouse IL-17C Reporter Cellshkb-il17c
HEK-Blue™ IL-18 Cells Human & Mouse IL-18 Sensor Cellshkb-hmil18
HEK-Blue™ IL-22 Cells Human & Mouse IL-22 Reporter Cellshkb-il22
HEK-Blue™ IL-23 Cells Human & Mouse IL-23 Reporter Cellshkb-il23
HEK-Blue™ IL-33 Cells Human IL-33 Sensor Cellshkb-hil33
Tumor Growth Factor Reporter Cells

Cell lineDescriptionCat no.
HEK-Blue™ TGF-β Cells Human TGFβ SEAP reporter HEK293 cellshkb-tgfb
Tumor Necrosis Factor Reporter Cells

Cell lineDescriptionCat no.
HEK-Blue™ TNF-α Cells Human TNF-α SEAP reporter HEK293 cells
NF-κB/AP-1 pathway
hkb-tnfdmyd
HEK-Dual™ TNF-α Cells Human and Murine TNF-α SEAP / luciferase reporter HEK293 cells - NF-κB pathwayhkd-TNFA
HEK-Blue™ CD40L Cells Human CD40L SEAP reporter HEK293 cellshkb-cd40
HEK-Blue™ RANKL Cells Human & Mouse RANKL Reporter Cellshkb-rankl
HEK NF-κB Reporter Cells NF-kB SEAP Reporter HEK 293 - parental cell lines
Cell lineDescriptionCat no.
HEK-Blue™ Null1 Cells NF-kappaB / SEAP parental cell linehkb-null1
HEK-Blue™ Null1-k cells NF-kappaB / SEAP parental cell linehkb-null1k
HEK-Blue™ Null1-v cells NF-kappaB / SEAP parental cell linehkb-null1v
HEK-Blue™ Null2-k cells NF-kappaB / SEAP parental cell linehkb-null2k
HEK-Blue™ Null2 cells NF-kappaB / SEAP parental cell linehkb-null2


HeLa-Difluo™ hLC3 cells : Human HeLa cervix carcinoma - Autophagy reporter cells

Cell lineDescriptionCat no.
HeLa-Difluo™ hLC3 cellsAutophagy Reporter Cellsheldf-hlc3


HepG2 hepatoma [NEW] : Human HepG2 liver carcinoma - Transcription factor reporter cells

Cell lineDescriptionCat no.
HepG2-Dual™ Cells NFkB-SEAP & IRF-Lucia reporter liver carcinoma cellshepg2d-nfis
HepG2-Lucia™ AhR Cells [NEW] Human HepG2 liver carcinoma - AhR-Lucia reporter cellshpgl-AHR


HT29-Lucia™ AhR Cells [NEW] : Human HT29 colon adenocarcinoma - AhR reporter cells

Cell lineDescriptionCat no.
HT29-Lucia™ AhR CellsHuman HT29 colon adenocarcinoma - AhR reporter cellsht2l-AHR


J774-Dual™ Cells : Murine J774.1 macrophage-like cells - NF-κB & IRF reporter cells

Cell lineDescriptionCat no.
J774-Dual™ CellsMurine NF-kB & IRF Reporter macrophage-like cellsj774d-nfis


Jurkat T Lymphocytes : Human Jurkat T Lymphocytes - Transcription factor reporter cells

Cell lineDescriptionCat no.
Jurkat-Dual™ Cells Human T Lymphocytes - NF-kB/IRF reporter cellsjktd-isnf
Jurkat-Lucia™ NFAT Cells NFAT-Luc Reporter T Lymphocytesjktl-nfat
Jurkat-Lucia™ NFAT-CD16 Cells Human T Lymphocytes - ADCC Reporter Cellsjktl-nfat-cd16


Ramos B lymphocytes : Human Ramos B lymphocytes - NF-κB & AP-1 reporter cells

Cell lineDescriptionCat no.
Ramos-Blue™ Cells NF-kB/AP-1 reporter B lymphocytesrms-sp
Ramos-Blue™ KD-MyD Cells NF-κB/AP-1 SEAP reporter B lymphocytes with MyD88 knockdownrms-kdmyd


RAW 264.7 macrophages : Murine RAW 264.7 macrophages - PRR or cytokine reporter cells

NF-κB-Reporter Cells

Cell lineDescriptionCat no.
RAW-Blue™ Cells NF-κB-SEAP Reporter Cell Lineraw-sp
IRF-Reporter Cells

Cell lineDescriptionCat no.
RAW-Blue™ ISG Cells IRF-SEAP-Reporter Cell Lineraw-isg
RAW-Lucia™ ISG Cells IRF-Lucia Luciferase Reporter Cell Linerawl-isg
IRF/KIN-MIP2 -Reporter Cells

Cell lineDescriptionCat no.
RAW-Dual™ Cells Dual IRF and MIP-2 (NF-kB) reporter mouse macrophagesrawd-ismip
Knockout Cells

Cell lineDescriptionCat no.
RAW-Dual™ KO-TLR4 Cells TLR4 knockout IRF and MIP-2 (NF-κB) reporter mouse macrophagesrawd-kotlr4
RAW-Lucia™ ISG-KO-cGAS Cells Murine RAW 264.7 macrophages - cGAS Knockout IRF-reporter cellsrawl-kocgas
RAW-Lucia™ ISG-KO-IFI16 Cells Murine RAW 264.7 macrophages - IFI16 Knockout IRF-reporter cellsrawl-koif16
RAW-Lucia™ ISG-KO-IRF3 Cells Murine RAW 264.7 macrophages - IRF3 Knockout IRF-reporter cellsrawl-koirf3
RAW-Lucia™ ISG-KO-IRF7 Cells IRF7 Knockout IRF-Lucia Luciferase Reporter Cell Linerawl-koirf7
RAW-Lucia™ ISG-KO-IRF5 CellsMurine RAW 264.7 macrophages - IRF5 knockout and IRF-Lucia Reporter Cellsrawl-koirf5
RAW-Lucia™ ISG-KO-MAVS Cells MAVS Knockout IRF-Lucia Luciferase Reporter Cellsrawl-komavs
RAW-Lucia™ ISG-KO-MDA5 Cells MDA-5 Knockout IRF-Lucia Luciferase Reporter Cell Linerawl-komda5
RAW-Lucia™ ISG-KO-RIG-I Cells RIG-I Knockout IRF-Lucia Luciferase Reporter Cell Linerawl-korigi
RAW-Lucia™ ISG-KO-STING Cells Murine RAW 264.7 macrophages - STING Knockout IRF-reporter cellsrawl-kostg
RAW-Lucia™ ISG-KO-TBK1 Cells Murine RAW 264.7 macrophages - TBK1 Knockout IRF-reporter cellsrawl-kotbk
RAW-Lucia™ ISG-KO-TREX1 Cells TREX1 Knockout IRF-Lucia Luciferase Reporter Cell Linerawl-kotrex
RAW-Lucia™ ISG-KO-TRIF Cells TRIF Knockout IRF-Lucia Luciferase Reporter Cell Linerawl-kotrif
Autophagy Reporter Cells

Cell lineDescriptionCat no.
RAW-Difluo™ mLC3 Cells Autophagy Reporter Cellsrawdf-mlc3


THP-1 monocytes : Human THP-1 monocytes - PRR reporter cells

THP-1 monocytes Human THP-1 monocytes - PRR reporter cells
Cell lineDescriptionCat no.
THP1-Dual™ KI-mSTING CellsMurine STING knockin NF-kB-SEAP and IRF-Lucia Luciferase Reporter Monocytesthpd-mstg



NF-κB Reporter Monocytes

Cell lineDescriptionCat no.
THP1-Blue™ NF-κB Cells Human THP-1 Monocytes - NF-kB SEAP Reporterthp-nfkb
THP1-Lucia™ NF-κB Cells Human THP-1 Monocytes - NF-κB Luc Reporter Monocytesthpl-nfkb
IRF Reporter Monocytes

Cell lineDescriptionCat no.
THP1-Blue™ ISG Cells Human THP-1 Monocytes - IRF SEAP Reporterthp-isg
THP1-Lucia™ ISG Cells Human THP-1 Monocytes - IRF Luciferase Reporter Cellsthpl-isg
THP1-Blue™ KI-IP10 Cells [NEW] Human THP-1 Monocytes - ISG Luciferase Reporter Cellsthpb-ip10kilc
NF-κB and IRF Reporter Monocytes
Cell lineDescriptionCat no.
THP1-Dual™ Cells Human THP-1 Monocytes - NF-κB-SEAP IRF-Luc Reporterthpd-nfis
THP1-Dual™ KO-cGAS Cells Human THP-1 Monocytes - cGAS knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-kocgas
THP1-Dual™ KO-IFI16 Cells Human THP-1 Monocytes - IFI16 knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-koifi16
THP1-Dual™ KO-IFNAR2 Cells Human THP-1 Monocytes - IFNAR2 knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-koifnar2
THP1-Dual™ KO-MyD Cells Human THP-1 Monocytes - MyD88 knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-komyd
THP1-Dual™ KO-STING Cells Human THP-1 Monocytes - STING knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-kostg
THP1-Dual™ KI-hSTING-S154 Cells Human THP-1 Monocytes - STING (S154 isoform; constitutively active) knockin NF-kB-SEAP and IRF-Lucia Reporter Cellsthpd-s154
THP1-Dual™ KI-hSTING-M155 Cells Human THP-1 Monocytes - STING (M155 isoform; constitutively active) knockin NF-kB-SEAP and IRF-Lucia Reporter Cellsthpd-m155
THP1-Dual™ KI-hSTING-A162 Cells Human THP-1 Monocytes - STING (A162 isoform) knockin NF-kB-SEAP and IRF-Lucia Reporter Cellsthpd-a162
THP1-Dual™ KI-hSTING-H232 Cells Human THP-1 Monocytes - STING (H232 isoform) knockin NF-kB-SEAP and IRF-Lucia Reporter Cellsthpd-h232
THP1-Dual™ KI-hSTING-R232 Cells Human THP-1 Monocytes - STING (R232 isoform) knockin NF-kB-SEAP and IRF-Lucia Reporter Cellsthpd-r232
THP1-Dual™ KO-TBK1 CellsHuman THP-1 Monocytes - TBK1 knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-kotbk
THP1-Dual™ KO-TREX1 Cells Human THP-1 Monocytes - TREX1 knockout NF-κB-SEAP and IRF-Lucia Reporter Cellsthpd-kotrex
Inflammasome Test Cells

Cell lineDescriptionCat no.
THP1-ASC-GFP Cells Human THP-1 Monocytes - ASC speck reporter cellsthp-ascgfp
THP1-defASC Cells Human THP-1 Monocytes - ASC-deficientthp-dasc
THP1-defCASP1 Cells Human THP-1 Monocytes - caspase-1 deficientthp-dcasp1
THP1-defNLRP3 Cells Human THP-1 Monocytes - NLRP3-deficientthp-dnlp
THP1-HMGB1-Lucia™ Cells Pyroptosis and necroptosis reporter monocytesthp-gb1lc
THP1-NLRC4 Cells Human THP-1 Monocytes - NLRC4 inflammasome testthp-nlrc4
THP1-Null Cells Human THP-1 Monocytes - Positive Control for Inflammasome Studiesthp-null
Autophagy Reporter Cells

Cell lineDescriptionCat no.
THP1-Difluo™ hLC3 Cells Autophagy reporter cellsthpdf-hlc3


- InvivoGen 에서 판매하는 cell line은 LMO 신고 후에 구매가 가능합니다.

- 모든 세포는 실온(flask)으로 배송되고 있습니다. -

- 학술 문의   T.  031-728-3236   E.  technical@dawinbio.com


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