Antibodies & ELISAsIn vivo 마우스 실험을 위한 Mouse Anti-Mouse mAbs

2022-04-18
조회수 4943



Mouse Anti-Mouse mAbs

In vivo 마우스 실험을 위한 Recombinant monoclonal antibody

InvivoGen은 mouse에 반복적인 injection 시 immunogenicity (면역원성)을 제한하도록 설계된 InvivoFit™ recombinant mouse 혹은 Murinized anti-mouse monoclonal antibody (mAb)를 제공합니다.

InvivoGen의 mAb는 Chinese hamster ovary (CHO) cell에서 생산됩니다. In vivo 마우스 실험에 사용 가능한 high-quality의 InvivoFit™ 등급의 아래와 같은 단백질을 타겟하는 mAb를 제공합니다.

  • Cytokines: mIL-1α, mIL-1β, mIL-6, mIL-13, and mIL-28B
  • Immune checkpoints : mCTLA-4, mPD-1, mPD-L1, and mTIGIT
  • Lymphocyte markers : mCD20, mCD25, mCD3, mCD4, and mCD8 
  • Tumor-associated antigens : gp75
  • E. coli β-galactosidase for isotype controls

* Key Features

  • Sequence : 65% to 100% murine
  • Guaranteed sterile, endotoxin level : <1 EU/mg
  • Suitable for parenteral delivery in mice (azide-free)
  • Low aggregation : < 5%
InvivoGen의 recombinant mouse와 murinized anti-mouse mAbs는 100% mouse origin (constant and variable regions) 혹은 ~65% mouse origin (constant regions) sequence인 항체를 제공합니다.

Xenogeneic sequence (이종 서열)의 최소 사용은 mAb의 immunogenicity를 제한합니다. mAb 활성을 평가하기 위한 murine in vivo 마우스 실험에서 반복적인 injection 시 직면하는 주요 문제는 mouse origin의 sequence가 아닌 경우 발생하는 항체 성능의 감소 및 손실입니다. 

Mouse에 반복적인 injection은 host에 의한 anti-species antibody 생성을 유도합니다 (예 : mAb가 rat에서 생성된 경우 anti-rat antibody). 이러한 현상은 mouse가 아닌 다른 종 (host)의 sequence 비율이 높을수록 증가합니다.

Xenogeneic sequence를 mouse sequence로 대체할 경우 항체 immunogenicity가 감소하며, in vivo 환경에서 활성을 유지할 수 있습니다.


 * Ordering information

PRODUCTDESCRIPTIONQTYCAT. CODE
Cytokines
Anti-mIL-1α-mIgG1
Recombinant mouse mAb against mIL-1α (clone 6H7). For in vitro use.
200 µg
mil1a-mab9-02
Anti-mIL-1α-mIgG1 InvivoFit™ Recombinant mouse mAb against mIL-1α (clone 6H7). For in vivo use.
1mgmil1a-mab9-1
Anti-mIL-1β-mIgG1
Recombinant mouse mAb against mIL-1β (clone 7E3). For in vitro use.
200 µg
mil1b-mab9-02
Anti-mIL-1β-mIgG1 InvivoFit™
Recombinant mouse mAb against mIL-1β (clone 7E3). For in vivo use.
1mg / 10 mgmil1b-mab9-1/-10
Anti-mIL-6-mIgG1e3
Recombinant mouse mAb against mIL-6 (clone 10F9). For in vitro use.
200 µg
mil6-mab15-02
Anti-mIL-6-mIgG1e3 InvivoFit™
Recombinant mouse mAb against mIL-6 (clone 10F9). For in vivo use.
1mg / 10 mg

mil6-mab15-1/mil6-mab15-10

Anti-mIL-13-mIgG1
Recombinant mouse mAb against mIL-13 (clone 8H8). For in vitro use.
200 µg
mil13-mab9-02
Anti-mIL-13-mIgG1 InvivoFit™
Recombinant mouse mAb against mIL-13 (clone 8H8). For in vivo use.
1 mg
mil13-mab9-1
Anti-mIL-28b-mIgG1
Recombinant mouse mAb against mIL-28B (clone 3C11). For in vitro use.
200 µg
mil28b-mab9-02
Anti-mIL-28b-mIgG1 InvivoFit™
Recombinant mouse mAb against mIL-28B (clone 3C11). For in vivo use.
1 mg
mil28b-mab9-1
In vivo Lymphocyte markers mAbs
Anti-mCD3-mIgG2a InvivoFit™145-2C11-derived mouse mAb against murine CD31mg / 10 mgmcd3-mab10-1/-10
Anti-mCD4-mIgG2a InvivoFit™ GK1.5-derived mouse mAb against murine CD41mg / 10 mgmcd4-mab10-1/-10
Anti-mCD8-mIgG2a InvivoFit™ YTS169.4-derived mouse mAb against murine CD81mg / 10 mgmcd8-mab10-1/-10
Anti-mCD20-mIgG2a InvivoFit™18B12-derived mouse monoclonal antibody against murine CD201mg / 10 mgmcd20-mab10-1/-10
Anti-mCD25-PC-mIgG2a InvivoFit™PC-61.5.3-derived monoclonal antibody against murine CD25 (blocking)1mg / 10 mgmcd25c1-mab10-1/-10
Anti-mCD25-7D4-mIgG2a InvivoFit™7D4-derived monoclonal antibody against murine CD25 (non-blocking)1mg / 10 mgmcd25c2-mab10-1/-10
In vivo immune checkpoint blocking mAbs
Anti-mCTLA4-mIgG2a InvivoFit™9D9-derived mouse mAb against murine CTLA-41mg / 10 mgmctla4-mab10-1/-10
Anti-mPD-1-mIgG1e3 InvivoFit™RMP1-14-derived mouse mAb against murine PD-11mg / 10 mgmpd1-mab15-1/-10
Anti-PD-L1-mIgG1e3 InvivoFit™Murinized atezolizumab mouse mAb against PD-L11mg / 10 mgpdl1-mab15-1/-10
Anti-mTIGIT-mIgG2a InvivoFit™10A7-derived monoclonal antibody against murine TIGIT1mg / 10 mgmtigit-mab10-1/-10
In vivo tumor-associated antigen mAbs
Anti-mgp75-mIgG2a InvivoFit™TA99-derived mouse mAb against murine gp75 1mg / 10 mgmgp75-mab10-1/-10
In vivo isotype controls
Anti-β-Gal-mIgG1e3 InvivoFit™ Mouse IgG1e3 isotype control1mg / 10 mgbgal-mab15-1/-10
Anti-β-Gal-mIgG2a InvivoFit™ Mouse IgG2a isotype control1mg / 10 mgbgal-mab10-1/-10

[학술문의]  T. 031-728-3236 E. technical@dawinbio.com

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