RT-PCR편차 없는 cDNA 합성, amfiRivert cDNA Synthesis Platinum Master Mix와 함께하세요!

 제품 특징

  • UP TO 17.8Kb

         - Modified M-MLV RTase : 기존 M-MLV 대비 높은 활성 온도 (40-60℃) 를 가지며 

           RNase 활성이 낮아 긴 길이의 cDNA를 합성할 수 있음 (최대 17.8Kb)

  • 두 가지 vial로 구성 (편리하게 사용가능)

          - Enzyme Mix: amfiRivert Reverse Transcriptase와 RNase Inhibitor Plus가 

            포함되어 있음

          - 2x Buffer Mix: Oigo dT(18)와 random hexamer가 최적의 비율로 혼합되어 

           있어서 편차 없이 cDNA 합성이 가능하며, cDNA 합성에 필요한 요소 (MgCl2, dNTP 

           mixture) 가 모두 최적의 농도로 포함되어 있어서 추가적인 optimization이 필요 없음 

  • Wide range of RNA

           - 실험에 따라 RNA input을 유동적으로 조절 가능 

              (Total RNA: 10 pg ~5 ug, mRNA: 10 pg ~ 500 ng) 

 실험 데이터

Ordering information

 Product nameCat No.Size
amfiRivert cDNA Synthesis Platinum Master MixR5600-05050 rxns
R5600-1002x50 rxns
R5600-2004x50 rxns
R5600-50010x50 rxns

* 본 제품은 Sample test 가능합니다. (Click!)

* 제품 문의   

   T. 031-728-3237, E. technical@dawinbio.com

References (참고문헌)

  • Enhanced antitumor immunotherapeutic effect of B-cell-based vaccine transduced with modified adenoviral vector containing type 35 fiber structures Gene Therapy, November 2014
  • Induction of cancer-related microRNA expression profiling using excretory-secretory products of Clonorchis sinensis. Parasitology Research, September 2014
  • Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation. Biochimica et Biophysica Acta, May 2014
  • Expression of Airway Remodeling Proteins in Mast Cell Activated by TGF-β Released in OVA-Induced Allergic Responses and Their Inhibition by Low-Dose Irradiation or 8-oxo-dG Radiation Research, April 2014
  • Transforming Growth Factor-β1 Downregulates Vascular Endothelial Growth Factor-D Expression in Human Lung Fibroblasts via the Jun NH2-Terminal Kinase Signaling Pathway Molecular Medicine, February 2014
  • Mesenchymal stem cells enhance autophagy and increase β-amyloid clearance in Alzheimer disease models. Landes Bioscience, January 2014
  • Proatherogenic conditions promote autoimmune T helper 17 cell responses in vivo. PubMed, January 2014
  • The Requirement for p42/p44 MAPK Activity in Progesterone Receptor-Mediated Gene Regulation is Target Gene-Specific NIH, June 2013
  • Elevated Homocysteine by Levodopa Is Detrimental to Neurogenesis in Parkinsonian Model PLOS ONE, November 2012
  • Polycomb (PcG) Proteins, BMI1 and SUZ12, Regulate Arsenic-induced Cell Transformation The Journal of Biological Chemistry, September 2012
  • TCF7L2 Modulates Glucose Homeostasis by Regulating CREB- and FoxO1-Dependent Transcriptional Pathway in the Liver. PLOS Genetics, September 2012
  • A role for O-GlcNAcylation in setting circadian clock speed. Genes & Development, February 2012
  • Signal pathways in astrocytes activated by cross-talk between of astrocytes and mast cells through CD40-CD40L Journal of Neuroinflammation, March 2011
  • Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells. Respiratory Research, 2011
  • Elevated pressure, a novel cancer therapeutic tool for sensitizing cisplatin-mediated apoptosis in A549. Biochemical and Biophysical Research Communications, August 2010
  • Signaling Pathways in the Activation of Mast Cells Cocultured with Astrocytes and Colocalization of Both Cells in Experimental Allergic Encephalomyelitis The Journal of Immunology, May 2010