| Name | QIAquant 96 (2plex and 5plex) | QIAquant 384 5plex |
| Block format | 96 wells | 384 wells |
| Block materials | Silver sample block with gold coating | Aluminum, special alloy |
| Sample volume | 5–100 μl (10 to 80 μl recommended) | 2–30 μl (5 to 20 μl recommended) |
| Lid temperature | 30–110°C |
| Temperature gradient | 40°C (0.1°C steps) | 25°C (0.1°C steps) |
| Max. heating rate* | max. 8°C/s, av. 7°C/s
(depending on consumables used) | max. 4°C/s, av. 3.8°C/s
(depending on consumables used) |
|
| Max. cooling rate* | Max. cooling rate* max. 6°C/s, av. 5.5°C/s
(depending on consumables used) | max. 2°C/s, av. 1.7°C/s
(depending on consumables used) |
|
| Heating rate adjustment | min. 0.1°C/s |
Temperature uniformity
(15 s after starting the clock) | ±0.15°C at 55°C |
| ±0.25°C at 72°C |
| ±0.50°C at 95°C |
| Temperature range | 3°C – 99°C |
| Temperature accuracy | 0.1°C |
| Supported plastic products | 96-well micro titer plates with optical film,
8-well strips 0.2 ml with optical lids,
0.2 ml individual vessels with optical lids | 384-well PCR plates with optical sealing film |
|
|
| Sensitivity | 1 nmol/l FAM at 30 μl sample
volume in a 96-well PCR plate | 1 nmol/l FAM at 30 μl sample
volume in a 384-well PCR plate |
|
| Dynamic range | 10 log stages |
| Light source | Four high-intensity LEDs (blue, green, white, red) |
| Detector | Photomultiplier (PMT) |
| Analysis methods | Absolute quantification, relative quantification, △△Ct method, allelic discrimination, |
| efficiency calculation, DNA melting curves, POS/NEG analysis in the end point |
| Export functions | Excel, CSV, LIMS, GenEx, qBase |
■ QIAGEN > QIAquant
QIAquant는 expression analysis, genotyping, pathogen detection 등 다양한 실험에 적용 가능한 프리미엄 block type Real-Time PCR 장비인 QIAquant는 fiber-optic shuttle system을 적용하여 빠른 heating, cooling rate가 가능하며, block 안에서 편차 없는 온도 균일성을 제공하기 때문에 빠르고 정확한 실험이 가능합니다.
QIAquant real-time cyclers
• block type real time PCR : 96-well block or 384-well block type 중 선택 가능
• Multiplex detection : 2 plex or 5 plex different target detection 가능
• Fast ramping : Up to 8 °C /sec
• Assay optimization : Gradient Tool을 이용하여 간편하게 Assay optimization가능
• Fast cycling : 빠른 실험 결과 (40 cycles, 30분 안에 결과 확인 가능)
QIAquant Specification
(depending on consumables used)
(depending on consumables used)
(depending on consumables used)
(depending on consumables used)
(15 s after starting the clock)
8-well strips 0.2 ml with optical lids,
0.2 ml individual vessels with optical lids
volume in a 96-well PCR plate
volume in a 384-well PCR plate
Ordering Information
software, and touchscreen interface. 115V power specification.
software, and touchscreen interface. 230V power specification.
software, and touchscreen interface. 115V power specification.
software, and touchscreen interface. 230V power specification.
384-well block and software. 115V power specification.
384-well block and software. 230V power specification.