| WSE-7135 EzPreStain DNA&RNA |
| Major components |
| DMSO, fluorescent dye
|
| Preparation |
| ◆ Pre-staining
1. Add to agarose gel solution at a 1:10,000 ratio during gel preparation.
2. Dilute 1:10,000 in TAE, TBE, or TE and add to the running buffer.
◆ Post-staining
1. Prepare a 1:10,000 staining solution using TAE, TBE, or TE.
|
| Usage |
| ◆ Pre-staining
1. Perform electrophoresis with agarose gel containing EzPreStain DNA & RNA, then detect immediately.
2. Perform electrophoresis using running buffer containing EzPreStain DNA & RNA, then detect immediately.
◆ Post-staining
1. Immerse the gel in the prepared staining solution for 10–30 min after electrophoresis.
- No destaining required.
- Do not use glass containers (dye may adhere).
|
| Detection |
| Recommended Light Sources
Excitation (Ex): 250–320 nm (UV), 440–500 nm (Blue LED), 470–520 nm (Cyan LED)
Absorption peaks: 250 nm, 482 nm
Emission (Em): Max 509 nm
Recommended filter: 500–550 nm long-pass filter
|
| Package |
| 500 μL (10,000× concentrate)
|
Protein / Nucleic Acid Staining Selection Guide
* 제품명 클릭 시 상세 설명으로 이동합니다.
AQua
Silver
Reverse
AQua MEM
DNA
DNA&RNA
Nucleic acid (DNA/RNA)
* ssDNA 제외
Polyacrylamide
Polyacrylamide
Polyacrylamide
(blue)
(밴드 제외 흰색)
(blue)
1. EzStain AQua
Specification
Ordering Information
2. EzStain Silver
- 단백질: 밴드당 수 ng 수준
- 핵산 (DNA/RNA): 밴드당 수십 ng 수준
- 글루타르알데하이드(glutaraldehyde) 무함유
- 후속 Mass Spectrometry (MS) 분석에 적합
Specification
S-2: silver nitrate
S-3: sodium hydrate
S-4: sodium thiosulfate and formaldehyde
R-1: Boric acid, SDS
R-2: Zinc sulfate
Ordering Information
3. EzStain Reverse
- 단백질 밴드는 투명하게 유지
- 배경만 흰색으로 염색
- 단백질 밴드당 수 ng 수준까지 검출 가능
- Coomassie 염색법보다 약 수 배~10배 높은 감도
- 염색 제거 용이
- 단백질 밴드의 절취 (trimming) 및 회수 (recovery) 에 적합
Specification
R-2: Zinc sulfate
Place the gel in a tray filled with the appropriate amount of the solution and immersed in each solution in order
Ordering Information
4. EzStain AQua MEM
- 항체 반응 전 염색
- Transfer 상태 및 효율 확인
- 항체 반응 후 총 단백질 신호 확인 가능
- 타겟 단백질 정규화 가능
1) Membrane staining 결과
2) Staining 후 항체 반응
Specification
Stain (Staining solution): 500 mL
De-Stain (Destaining solution): 500 mL
Bleach (Compelete destaining solution): 500 mL
De-Stain: 2× dilute with methanol
Bleach: 2× dilute with methanol
20~25 sheets in mini size gel (80 × 95 mm)
Ordering Information
5. EzFluoroStain DNA
- Ames Test에서 최저 수준의 변이원성 결과 확인
- IARC(국제암연구소) 지정 발암물질 미함유
- 낮은 배경 신호 및 높은 감도
Specification
Peak of absorption wavelength: 270 nm, 370 nm, 497 nm
Fluorescence: Max absorption wavelength 522 nm
Filter: 500~550 nm LP filter
Ordering Information
6. EzPreStain DNA&RNA
- Gel 제조 시 시약 첨가하여 전기영동 직후 검출 가능
- Running buffer 에 첨가하여 전기영동 직후 검출 가능
- 전기영동 후 DNA/RNA 염색 가능 (TAE, TBE, TE buffer 사용)
- 1:10,000 희석하여 사용
Speicification
1. Add to agarose gel solution at a 1:10,000 ratio during gel preparation.
2. Dilute 1:10,000 in TAE, TBE, or TE and add to the running buffer.
◆ Post-staining
1. Prepare a 1:10,000 staining solution using TAE, TBE, or TE.
1. Perform electrophoresis with agarose gel containing EzPreStain DNA & RNA, then detect immediately.
2. Perform electrophoresis using running buffer containing EzPreStain DNA & RNA, then detect immediately.
◆ Post-staining
1. Immerse the gel in the prepared staining solution for 10–30 min after electrophoresis.
- No destaining required.
- Do not use glass containers (dye may adhere).
Excitation (Ex): 250–320 nm (UV), 440–500 nm (Blue LED), 470–520 nm (Cyan LED)
Absorption peaks: 250 nm, 482 nm
Emission (Em): Max 509 nm
Recommended filter: 500–550 nm long-pass filter
Ordering Information
제품 문의: (주)다윈바이오텍
Tel. 031-728-3237 E-mail. technical@dawinbio.com
#다윈바이오 #ATTO #Gel staining #Protein staining #Nucleic acid staining